COLD STAIN TB KIT FOR MYCOBACTERIA

This staining kit is based on the Ziehl-Neelsen stain (a carbofulfuchsin solution). It is use as as modified basic fuchsin stain (without heating) for the identification of acid-fast bacteria such as Mycobacterium tuberculosis Acid-fast bacteria such as Mycobacterium tuberculosis and Mycobacterium leprae are difficult to stain because of their lipoid capsule in the cell wall. However, once the lipoid capsule is stained with an enhanced dye such as carbolfuchsin, the newly formed compound will resist decolorization from acid-alcohol and retain the original color stained (red), which can be easily differentiated from other microorganisms that still adsorb the counter blue stain. Expected results: Acid-fast Mycobacterium species will appear bright red, while other bacteria and cells will be blue. Contains: Carbol Fuchsin Strong Staining Solution (02522) - 125 mL, Acid Fast Decolorizer (00585) - 125 mL, Methylene Blue Alkaline (Loeffler's) Staining Solution (04647) - 125mL