GIEMSA'S STAINING SOLUTION For Microscopy

GIEMSA'S STAINING SOLUTION For Microscopy

Synonyms : azur-eosin-methylene blue staining solution

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifier
Chemical Name : GIEMSA’S STAIN SOLUTION
Catalogue No. : AL2277 Product form : Mixture
CAS Number : EC Number :
Grade : EC Index Number :
Chemical Formula : HSN Code : 38220090
Synonyms : azur-eosin-methylene blue staining solution
Chemical Structure :
Industrial. For professional use only :
1.2 Relevant identified uses of the substance or mixture and uses advised against
Use of the substance/mixture : Industrial For professional use only
Uses advised against : No additional information available
1.3 Details of the supplier of the safety data sheet
Company identification : Address:- 102, B wing, Savgan Heights, RTO Lane,
Andheri (W), Mumbai-400 053
Maharashtra, India.
1.4 Emergency Contact Details
Phone / e-Mail : Phone : +91 22 26317055
Mobile : +91 9820385757
e-mail : info@alphachemika.co.in

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No. 1272/2008 [CLP]
Mixture/Substance: SDS EU 2015: According to Annex II
of Regulation (EC) No. 453/2010 (REACH Annex II)
Flammable liquids,
Category 2
H225
Acute toxicity (oral),
Category 3
H301
Acute toxicity (dermal),
Category 3
H311
Acute toxicity (inhal.),
Category 3
H331
Specific target organ
toxicity single
exposure, Category 1
H370
Full text of classification categories and H statements : see section 16
Adverse physicochemical, human health and environmental effects
No additional information available
2.2 Label elements
Labelling according to Regulation (EC) No. 1272/2008 [CLP]
Hazard pictograms (CLP) : GHS02GHS02 GHS06GHS06 GHS08GHS08
Signal word (CLP) : Danger
Hazard statements (CLP) : H225 - Highly flammable liquid and vapour
H301+H311+H331 - Toxic if swallowed, in contact with skin or if inhaled
H370 - Causes damage to organs
Precautionary statements (CLP) : P210 - Keep away from heat, hot surfaces, sparks, open flames and other ignition
sources. No smoking
P233 - Keep container tightly closed
P240 - Ground and bond container and receiving equipment
P241 - Use explosion-proof equipment
P260 - Do not breathe dust/fume/gas/mist/vapours/spray
P264 - Wash ... thoroughly after handling
P270 - Do not eat, drink or smoke when using this product
P271 - Use only outdoors or in a well-ventilated area
P280 - Wear protective gloves/protective clothing/eye protection/face protection
P301+P310 - IF SWALLOWED: Immediately call a POISON CENTER/doctor/¦
P302+P352 - IF ON SKIN: Wash with plenty of water/¦
P303+P361+P353 - IF ON SKIN (or hair): Take off immediately all contaminated
clothing. Rinse skin with water
P304+P340 - IF INHALED: Remove person to fresh air and keep comfortable for
breathing
P308+P311 - IF exposed or concerned: Call a POISON CENTER/doctor/¦
P311 - Call a POISON CENTER/doctor/¦
P321 - Specific treatment (see ... on this label)
P330 - Rinse mouth
P361+P364 - Take off immediately all contaminated clothing and wash it before
reuse
P370+P378 - In case of fire: Use ... to extinguish
P403+P233 - Store in a well-ventilated place. Keep container tightly closed
P403+P235 - Store in a well-ventilated place. Keep cool
P405 - Store locked up
P501 - Dispose of contents/container to {0|message= local/regional/national/international regulations>|default=hazardous or special waste
collection point, in accordance with local, regional, national and/or international
regulation|filter=(_)?DISPOSAL_.+}
2.3 Other hazards
Other hazards : No additional information available

SECTION 3: Composition/information on ingredients

3.1. Substance
Not applicable
3.2. Mixture
Name Product identifier % Classification according to Regulation (EC) No. 1272/2008 [CLP]
Methanol (CAS No) 67-56-1 (EC no) 200-659-6 (EC index no) 603-001-00-X 50 - 75 Flam. Liq. 2, H225 Acute Tox. 3 (Dermal), H311 Acute Tox. 3 (Inhalation), H331 Acute Tox. 3 (Oral), H301 STOT SE 1, H370
1,2,3-Propanetriol(glycerin) (CAS No) 56-81-5 (EC no) 200-289-5 50 - 75 Not classified
GIEMSA'S STAIN (CAS No) 51811-82-6 0.01 - 1 Eye Dam. 1, H318 Acute Tox. 4 (Oral), H302 Aquatic Chronic 3, H412
Full text of H-phrases: see section 16

SECTION 4: First aid measures

4.1 Description of first aid measures
First-aid measures after inhalation : Get medical advice/attention. Remove victim to fresh air and keep at rest in a
position comfortable for breathing.
First-aid measures after skin contact : Rinse skin with water/shower. Remove/Take off immediately all contaminated
clothing. Immediately call a POISON CENTER or doctor/physician. Wash with plenty
of soap and water. Specific measures (see ... on this label). Wash contaminated
clothing before reuse.
First-aid measures after eye contact : Remove contact lenses, if present and easy to do. Continue rinsing. Rinse cautiously
with water for several minutes. Get medical advice/attention. Obtain medical
attention if pain, blinking or redness persist.
First-aid measures after ingestion : Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention.
Immediately call a POISON CENTER or doctor/physician. Specific treatment (see ...
on this label).
4.2 Most important symptoms and effects, both acute and delayed
Symptoms relating to use : Symptoms/injuries : Causes damage to organs.
Symptoms/injuries after inhalation : Toxic if inhaled.
Symptoms/injuries after skin contact : Repeated exposure to this material can result in absorption through skin causing
significant health hazard. Toxic in contact with skin.
Symptoms/injuries after ingestion : Toxic if swallowed. Swallowing a small quantity of this material will result in serious
health hazard.
4.3 Indication of any immediate medical attention and special treatment needed
General information : Treat symptomatically.

SECTION 5: Fire-fighting measures

5.1 Extinguishing media
Suitable extinguishing media : Water spray. Foam. Dry powder. Carbon dioxide. Sand.
Unsuitable extinguishing media : Do not use a heavy water stream.
5.2 Special hazards arising from the substance or mixture
Fire hazard : Flammable liquid and vapour. Highly flammable liquid and vapour.
Explosion hazard : May form flammable/explosive vapour-air mixture.
5.3 Advice for firefighters
Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory
protection.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures
General measures : Remove ignition sources. No naked lights. No smoking. Use special care to avoid
static electric charges.
6.1.1. For non-emergency personnel
Emergency procedures : Evacuate unnecessary personnel.
6.1.2. For emergency responders
Protective equipment Use personal protective equipment as required
Equip cleanup crew with proper protection Ventilate area.
6.2 Environmental precautions
Avoid release to the environment. Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters.
6.3 Methods and material for containment and cleaning up
Methods for cleaning up : Collect spillage. On land, sweep or shovel into suitable containers. Soak up spills
with inert solids, such as clay or diatomaceous earth as soon as possible. Store
away from other materials.
6.4 Reference to other sections
Reference : See Heading 8. Exposure controls and personal protection.

SECTION 7: Handling and storage

7.1 Precautions for safe handling
Additional hazards when processed : Handle empty containers with care because residual vapours are flammable.
Precautions for safe handling : Avoid contact with skin and eyes. Do not breathe vapours. Provide good ventilation in process area to prevent formation of vapour. Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work. No
Hygiene measures : Do not eat, drink or smoke when using this product. Wash ... thoroughly after handling.
7.2 Conditions for safe storage, including any incompatibilities
Technical measures : Proper grounding procedures to avoid static electricity should be followed.
Ground/bond container and receiving equipment. Use explosion-proof
electrical/ventilating/lighting/… equipment.
Storage conditions : Keep container tightly closed. Keep in fireproof place. Keep only in the original
container in a cool, well ventilated place away from :
Incompatible products : Strong bases. Strong acids.
7.3 Specific end use(s)
No additional information available

SECTION 8: Exposure controls/personal protection

8.1 Control parameters
No additional information available
8.2 Exposure controls
Hand protection : Protective gloves. Wear protective gloves
Eye protection : Chemical goggles or safety glasses
Skin and body protection : Wear suitable protective clothing
Respiratory protection : Where exposure through inhalation may occur from use, respiratory protection equipment is recommended Other information : Do not eat, drink or smoke during use.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
Physical state : Liquid Colour
Colour : No data available.
Odour : characteristic.
Odour threshold : No data available
pH value : 7
Relative evaporation rate (butylacetate=1) : No data available
Melting point : 17.9 °C
Freezing point : No data available
Boiling point : 64.5 °C
Flash point : 12 °C
Auto-ignition temperature : No data available
Decomposition temperature : No data available
Flammability (solid, gas) : Flammable Highly flammable liquid and vapour
Vapour pressure : 97.68 mm Hg at20°C)
Relative vapour density at 20°C : 2.37
Density : 1.04 g/cm3
Solubility : Water: Easily soluble in cold water
Log Pow : No data available
Viscosity, kinematic : No data available
Viscosity, dynamic : No data available
Explosive properties : No data available
Oxidising properties : No data available
Explosive limits : 6 - 36.5 vol %
9.2 Other information
No additional information available

SECTION 10: Stability and reactivity

10.1 Reactivity
No additional information available
10.2 Chemical stability
Flammable liquid and vapour. May form flammable/explosive vapour-air mixture. Highly flammable liq
10.3 Possibility of hazardous reactions
Not established.
10.4 Conditions to avoid
Open flame. Heat. Sparks. Overheating. Direct sunlight. Extremely high or low temperatures.
10.5 Incompatible materials
Strong acids. Strong bases.
10.6 Hazardous decomposition products
May release flammable gases. fume. Carbon monoxide. Carbon dioxide.

SECTION 11: Toxicological information

11.1 Information on toxicological effects
Acute toxicity : Oral: Toxic if swallowed. Dermal: Toxic in contact with skin. Inhalation: Toxic if
inhaled.
ATE CLP (oral) 100 mg/kg bodyweight
ATE CLP (dermal) 300 mg/kg bodyweight
ATE CLP (gases) 700 ppmv/4h
ATE CLP (vapours) 3 mg/l/4h
ATE CLP (dust,mist) 0.5 mg/l/4h
Skin corrosion/irritation : Not classified
pH: 7
Additional information : Based on available data, the classification criteria are not met
Serious eye damage/irritation : Not classified
pH: 7
Additional information : Based on available data, the classification criteria are not met
Respiratory or skin sensitisation : Not classified
Additional information : Based on available data, the classification criteria are not met
Germ cell mutagenicity : Not classified
Based on available data, the classification criteria are not met
Carcinogenicity : Not classified
Additional information : Based on available data, the classification criteria are not met
Reproductive toxicity : Not classified
Additional information : Based on available data, the classification criteria are not met
Specific target organ toxicity (single exposure) : Causes damage to organs.
Specific target organ toxicity (repeated exposure) : Not classified Additional information
Aspiration hazard : Not classified
Additional information : Based on available data, the classification criteria are not met
Potential adverse human health effects and symptoms : Toxic if swallowed. Toxic in contact with skin.

SECTION 12: Ecological information

12.1 Toxicity
Toxicity information : No additional information available
12.2 Persistence - degradability
Persistence - degradability : Giemsa's Staining Solution for Microscopy Persistence and degradability Not established.
12.3 Bioaccumulative potential
Bioaccumulative potential : Giemsa's Staining Solution for Microscopy Bioaccumulative potential Not established.
12.4 Mobility in soil
Mobility in soil : No additional information available
12.5 Results of PBT and vPvB assessment
No additional information available
12.6 Other adverse effects
Environmental precautions : Additional information : Avoid release to the environment

SECTION 13: Disposal considerations

13.1 Waste treatment methods
Product/Packaging disposal recommendations : Dispose in a safe manner in accordance with local/national regulations. Dispose of contents/container to ...
Additional information : Handle empty containers with care because residual vapours are flammable.
Ecology - waste materials : Avoid release to the environment. Hazardous waste due to toxicity.

SECTION 14: Transport information

14.1. UN number
UN-No. (ADR) : 1230
UN-No. (IMDG) : 1230
UN-No. (IATA) : 1230
UN-No. (ADN) : 1230
UN-No. (RID) : 1230
14.2. UN proper shipping name
Proper Shipping Name (ADR) : METHANOL
Proper Shipping Name (IMDG) : METHANOL
Proper Shipping Name (IATA) : METHANOL
Proper Shipping Name (ADN) : METHANOL
Proper Shipping Name (RID) : METHANOL
Transport document description (ADR) : UN 1230, METHANOL, 3 (6.1), II
Transport document description (IMDG) : UN 1230, METHANOL, 3 (6.1), II
Transport document description (IATA) : UN 1230, METHANOL, 3 (6.1), II
Transport document description (ADN) : UN 1230, METHANOL, 3 (6.1), II
Transport document description (RID) : UN 1230, METHANOL, 3 (6.1), II
14.3. Transport hazard class(es)
ADR
Transport hazard class(es) : 3 (6.1)
Danger labels : 3, 6.1
IMDG
Transport hazard class(es) : 3 (6.1)
Danger labels : 3, 6.1
IATA
Transport hazard class(es) : 3 (6.1)
Danger labels : 3, 6.1
ADN
Transport hazard class(es) : 3 (6.1)
Danger labels : 3, 6.1
RID
Transport hazard class(es) : 3 (6.1)
Danger labels : 3, 6.1
14.4. Packing group
Packing group (ADR) : II
Packing group (IMDG) : II
Packing group (IATA) : II
Packing group (ADN) : II
Packing group (RID) : II
14.5. Environmental hazards
Dangerous for the environment : No
Marine pollutant : No
Other information : No supplementary information available
14.6. Special precautions for user
- Overland transport
Classification code (ADR) : FT1
Special provision (ADR) : 279
Limited quantities (ADR) : 1L
Excepted quantities (ADR) : E2
Packing instructions (ADR) : P001, IBC02
Mixed packing provisions (ADR) : MP19
Portable tank and bulk container
instructions (ADR)
: T7
Portable tank and bulk container special
provisions (ADR)
: TP2
Tank code (ADR) : L4BH
Tank special provisions (ADR) : TU15
Vehicle for tank carriage : FL
Transport category (ADR) : 2
Special provisions for carriage - Loading,
unloading and handling (ADR)
: CV13, CV28
Special provisions for carriage - Operation
(ADR)
: S2, S19
Hazard identification number (Kemler No.) : 336
Orange plates :
Tunnel restriction code (ADR) : D/E
EAC code : •2WE
APP code : A(fl)
- Transport by sea
Special provision (IMDG) : 279
Limited quantities (IMDG) : 1 L
Excepted quantities (IMDG) : E2
Packing instructions (IMDG) : P001
IBC packing instructions (IMDG) : IBC02
Tank instructions (IMDG) : T7
Tank special provisions (IMDG) : TP2
EmS-No. (Fire) : F-E
EmS-No. (Spillage) : S-D
Stowage category (IMDG) : B
Stowage and handling (IMDG) : SW2
Flash point (IMDG) : 12°C c.c.
MFAG-No : 131
- Air transport
PCA Excepted quantities (IATA) : E2
PCA Limited quantities (IATA) : Y341
PCA limited quantity max net quantity
(IATA)
: 1L
PCA packing instructions (IATA) : 352
PCA max net quantity (IATA) : 1L
CAO packing instructions (IATA) : 364
CAO max net quantity (IATA) : 60L
Special provision (IATA) : A104, A113
ERG code (IATA) : 3L
- Inland waterway transport
Classification code (ADN) : FT1
Special provisions (ADN) : 279, 802
Limited quantities (ADN) : 1 L
Excepted quantities (ADN) : E2
Carriage permitted (ADN) : T
Equipment required (ADN) : PP, EP, EX, TOX, A
Ventilation (ADN) : VE01, VE02
Number of blue cones/lights (ADN) : 2
- Rail transport
Classification code (RID) : FT1
Special provision (RID) : 279
Limited quantities (RID) : 1L
Excepted quantities (RID) : E2
Packing instructions (RID) : P001, IBC02
Mixed packing provisions (RID) : MP19
Portable tank and bulk container
instructions (RID)
: T7
Portable tank and bulk container special
provisions (RID)
: TP2
Tank codes for RID tanks (RID) : L4BH
Special provisions for RID tanks (RID) : TU15
Transport category (RID) : 2
Special provisions for carriage – Loading
and unloading (RID)
: CW13, CW28
Colis express (express parcels) (RID) : CE7
Hazard identification number (RID) : 336
14.7. Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code
Not applicable

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture
15.1.1 EU-Regulations :
Contains no substances with Annex XVII restrictions
Contains no REACH candidate substance
Contains no REACH Annex XIV substances.
15.1.2. National regulations
Germany
AwSV/VwVwS Annex reference : Water hazard class (WGK) 2, hazardous to water (Classification according to AwSV,
Annex 1)
12th Ordinance Implementing the Federal
Immission Control Act - 12.BImSchV
: Is not subject of the 12. BlmSchV (Hazardous Incident Ordinance)
Denmark
Class for fire hazard : Class I-1
Store unit : 1 liter
Classification remarks : F ; Emergency management guidelines for the storage of flammable
liquids must be followed
Recommendations Danish Regulation : Young people below the age of 18 years are not allowed to use the product
Pregnant/breastfeeding women working with the product must not be in direct
contact with the product
15.2 Chemical Safety Assessment
No chemical safety assessment has been carried out

SECTION 16: Other Information

Data sources : REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF
THE COUNCIL of 16 December 2008 on classification, labelling and packaging of
substances and mixturejs, am